This webinar introduces the Tumor Microenvironment (TME) Pathway in QIAGEN’s Ingenuity Pathway Analysis (IPA), demonstrating how users can explore immune and tumor interactions by constructing networks around targets like IL1B and predicting downstream effects on functions such as tumor proliferation. Using tools like Molecule Activity Predictor and Path Explorer, we show how curated literature-derived relationships can model cytokine activity and TME behavior. Additional features like BioProfiler, Land Explorer, and Activity Plot allow users to analyze public datasets (e.g., TCGA) for expression trends and regulatory roles, with use cases including PD-1–positive and –negative CD8+ T cells in lung cancer. IPA’s upstream regulator analysis further enabled prediction of key drivers influencing immune and tumor cell dynamics.