Tutorials
Understanding the P-Value of Overlap Statistic in IPA
8,741 views
Learn how to interpret your p-value of overlaps results in IPA. You can gain a deeper understanding of the right-tailed Fisher’s Exact Test, why it’s used in IPA, how the results are calculated, and caveats to keep in mind when interpreting your results.
For more information visit our website
Sections:
For more information visit our website
Sections:
0:00-1:09 Introduction to the video. The two types of p-values that are relevant for IPA and how they differ.
1:09-2:25 History of the Fisher’s Exact Test: the “Lady Tasting Tea” experiment.
2:25-5:50 Hypothesis testing for enrichment in IPA. The reference set, the null hypothesis used, and the overlap of molecules defined.
5:51-7:58 Why the right-tailed Fisher’s Exact Test is used in IPA.
7:58- 8:41 Explanation of sampling and expected proportions from the hypergeometric distribution.
8:41-9:34 Defining significance levels in the distribution. Why only the right-tailed, rather than two-tailed test, is considered in IPA.
9:35-11:04 The p-value defined and default significance level used in IPA.
11:04-11:12 How the p-value is calculated. How data is entered into the 2 x 2 contingency table and the formula used.
11:13-13:34 Example calculation and interpretation using the “Lady Tasting Tea” experimental data. Why the p-value of overlap calculations are Exact.
13:35-15:09 Transcriptomics example: Number of molecules tested and how they are categorized.
15:10-16:10 Transcriptomics example: data in 2 x2 contingency table, p-value calculation performed and interpreted.
16:11-18:07 Caveats for using and interpreting the p-value of overlap statistic in IPA.
18:08-18:36 Where to find more information about IPA and Customer Support information.
Related videos
Tutorials
Getting started
Learn how to get started using CLC Genomics workbench and CLC Main Workbench....
Tutorials
Data Formatting in IPA
Learn how to format your dataset for upload and subsequent analysis in IPA....
QIAGEN IPA
Data Upload in IPA
Learn how to upload your dataset into IPA. In this video tutorial, you will...
Tutorials
RNA-seq analysis part I
Creation of an RNA-seq sample and initial interpretation of the result in CLC...