QIAGEN CLC Genomics

Metatranscriptome analysis, annotation and pathways investigations using CLC Genomics Workbench

185 views
September 15, 2022
Using CLC Genomics Workbench, we will go through a pipeline for analyzing metatranscriptome NGS data from microbial communities and perform pathways interpretation on it.

· Import "raw" NGS sequencing data and prepare the samples for analysis

· Find relevant annotated genomes with a curated reference database while removing ribosomal RNA with the SILVA database (database of rRNAs)

· De novo assemble the unclassified reads into contigs to also find transcripts of taxa not present in the reference database

· Map the reads to the assembled contigs and database

· Build a functional profile of the samples to get an abundance of GO-terms.

· Statistical analysis of the groups

· Pathway analysis on the differential abundance analysis using MetaCyc database.

Related videos

QIAGEN CLC Genomics

Multi-locus sequence typing (MLST) using QIAGEN CLC Genomics Workbench

113 views October 12, 2022

QIAGEN CLC Genomics Workbench Premium provides tools and workflows for a...

QIAGEN CLC Genomics

Isolate Typing, Strain Identification and Anti-Microbial Resistance Analyses Using CLC Genomics...

169 views June 24, 2022

QIAGEN CLC Genomics Workbench provides tools and workflows for a broad range...

QIAGEN CLC Genomics

Pan-viral genomics analysis and interpretation

262 views March 31, 2022

Take your viral analysis to the next level. In this webinar we will guide you...

QIAGEN CLC Genomics

Isolate typing, strain identification and antimicrobial resistance analyses using QIAGEN CLC...

491 views August 26, 2021

QIAGEN CLC Genomics Workbench is widely used to analyze bacterial, viral and...